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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in many tumors, which results in chromosome instability, and so may possibly contribute to tumorigenesis. This protein was uncovered to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial job in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already explained. Two further splice variants had been claimed but in depth nucleotide sequence information will not be nevertheless accessible.
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Blocking peptides are peptides that bind particularly into the focus on antibody and block antibody binding. These peptide normally is made up of the epitope recognized because of the antibody.
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This guideline outlines further doable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide not bind to the epitope on the target protein. By evaluating the staining with the blocked antibody as opposed to the antibody by yourself, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterized by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means click here of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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